Tehran University of Medical Sciences The Research in Heart Yield and Translational Medicine (RHYTHM) 3115-7270 8 2 2015 10 13 89 94 EN Leila Poorgholi Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Hana Saffar Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Mahmood Sheikh-Fathollahi Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Gholamreza Davoodi Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Maryam Sotoudeh-Anvari Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Hamidreza Goodarzynejad Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Shayan Ziaee Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Ali Boroumand Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 11 2015 10 11  Background: The study of the association between genotype and phenotype is of great importance for the prediction of many diseases and pathophysiological conditions. The relationship between angiotensin-converting enzyme (ACE) gene insertion/ deletion (I/D) polymorphism and pathological processes such as coronary artery disease (CAD) has been investigated previously with discordant results. This study was designed to determine the association between ACE gene I/D polymorphism and CAD in an Iranian population. Methods: A total of 1050 individuals who were referred to Tehran Heart Center for coronary angiography were recruited. Six hundred seventy-six CAD-positive patients (documented by coronary angiography and Gensini scores higher than 6) and 374 CAD-negative patients were evaluated for ACE gene I/D polymorphism via the Polymerase Chain Reaction Amplification method. The patients’ age, sex, smoking status and its duration as well as familial history of CAD, hypertension, and diabetes mellitus were recorded. Results: Five hundred four (74.6%) of the CAD-positive patients were male, and the mean age of this group was 60 (60 ±10). In the CAD-negative individuals, the mean age was 56 (56 ± 10) and 196 of them were male (52.4%). After the analysis of all the groups and gender subgroups, neither genotype nor allele frequency was significantly different between the CAD- positive and CAD-negative groups (p values for genotypes and allele frequencies were 0.494 and 0.397, respectively). Conclusion: ACE gene I/D polymorphism was not associated with an increased risk of CAD in an Iranian population. https://rhythm.tums.ac.ir/index.php/jthc/article/view/288 https://rhythm.tums.ac.ir/index.php/jthc/article/download/288/281